Antiapoptotic Actions of Methyl Gallate on Neonatal Rat Cardiac Myocytes Exposed to H₂O₂

Figure 1

Oxidative stress affects viability and morphology of neonatal rat cardiac myocytes. LD₅₀ concentrations were determined for RCMs using varying concentrations of H₂O₂ (a) or CoCl₂ (b). RCMs, pretreated with 5 mM NAC, 100 μM EGCG, 50 μM GA, or 50 μM MG, were exposed to 800 μM H₂O₂ or 900 μM CoCl₂ for 24 hours before proceeding to MTT assay (c) or imaging (d) as described in the methods. Stressed cells versus unstressed controls (^†††𝑃<0.001) and versus stressed cells pretreated with NAC or polyphenols (***𝑃<0.001, *𝑃<0.05). Data is expressed as mean ± S.E.M., 𝑛=3.