SIRT1 Functions as an Important Regulator of Estrogen-Mediated Cardiomyocyte Protection in Angiotensin II-Induced Heart Hypertrophy

Figure 1

17-Estradiol (E2) elevates endogenous SIRT1 expression and attenuates Angiotensin II-induced heart hypertrophy. (a) The uterus weight and body weight ratio in mice that were sham-operated (sham), ovariectomized (Ovx), ovariectomized with Angiotensin II (AngII) infusion (Ovx + AngII), or ovariectomized with E2 replacement and AngII infusion (Ovx + AngII + E2) in vivo (, versus sham, ^## versus Ovx or Ovx + AngII group). (b) Mouse heart samples (5x), heart weight, and tibia length ratio of sham, Ovx, Ovx + AngII, and Ovx + AngII + E2 mice (, , ). (c)-(d) H&E staining of mouse heart samples and cardiomyocyte size statistical analysis (, magnification bar = 100 μm). (e) ANF, -MHC, and SIRT1 mRNA expression in mouse heart as assayed by real-time PCR (, versus sham, versus sham, ^# versus Ovx + AngII, and ^## versus Ovx + AngII). (f) SIRT1 expression in mouse heart as assayed by Western blot and the averaged data (, versus sham, versus sham). (g) The average data of ROS fluorescence intensity relative ratio in mouse heart analyzed by DHE staining (-6, ). (h) The average data from TUNEL staining of mouse heart samples (-6, , ).

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