Research Article
SIRT1 Functions as an Important Regulator of Estrogen-Mediated Cardiomyocyte Protection in Angiotensin II-Induced Heart Hypertrophy
Figure 2
Estrogen upregulates endogenous SIRT1 gene expression. (a) Representative H&E staining and the average cell surface area of NRVMs in control (Con), AngII, AngII + E2, and AngII + E2 + ICI (, ). (b)-(c) The average data of protein and DNA relative ratio (b) and ANF mRNA expression (c) in con, AngII, AngII + E2, and AngII + E2 + ICI groups of NRVMs (, , ). (d)-(e) SIRT1 expression in the 4 groups of NRVMs, as assayed by Western blot and the average data (, , ). (f) SIRT1 protein expression (assayed by Western blot) in the NRVMs treated with 1 × 10−10 M, 1 × 10−9 M, 1 × 10−8 M, and 1 × 10−7 M of E2 for 24 h (, versus control, versus the control).
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