PRAK Interacts with DJ-1 and Prevents Oxidative Stress-Induced Cell Death

Figure 7

PRAK helps DJ-1 to sequester Daxx in the nucleus. (a) PRAK^+/+ cells synchronized by serum starvation for 48 hrs and treated with culture medium (upper) or 300 μM H₂O₂ (lower) for 6 hrs were stained with antibodies against DJ-1 and Daxx and further visualized with FITC- and Texas red-conjugated secondary antibodies. (b) PRAK^−/− cells synchronized by serum starvation for 48 hrs and treated with culture medium (upper) or 300 μM H₂O₂ (lower) for 6 hrs were stained with antibodies against DJ-1 and Daxx and further visualized with FITC- and Texas red-conjugated secondary antibodies. Nuclei were stained with DAPI. (c) and (d). The nuclear and cytoplasmic fluorescence intensities of Daxx in PRAK^+/+ cells (c) and PRAK^−/− cells (d) were analyzed. Data are expressed as the mean ± SD of four separate experiments. compared with Daxx in the nucleus of naive or H₂O₂-treated PRAK^+/+ cells (c); compared with Daxx in the cytoplasm of naive PRAK^−/− cells (d).

(a)

(b)

(c)

(d)