Oxidative Stress Activates the Transcription Factors FoxO 1a and FoxO 3a in the Hippocampus of Rats Exposed to Low Doses of Ozone
Figure 4
Effect of ozone exposure on pFoxO 1a immunoreactivity in the rat hippocampus. (a) The photomicrographs show pFoxO 1a-positive cells (green) in the dentate gyrus (DG) region. Rats were exposed to ozone-free air (control) or 7, 15, 30, 60, and 90 days with ozone. A progressive increase can be observed in the immunoreactivity against pFoxO 1a from 15 days to 60 days of ozone exposure. Insets are a zoom from selected areas in the same picture. (b) A representative western blot shows the content of the total FoxO 1a and pFoxO protein in the hippocampi of rats exposed to ozone for different times. The graph shows densitometry values presented as the mean ± SD (∗).
