Polyol Pathway Exacerbated Ischemia/Reperfusion-Induced Injury in Steatotic Liver
Figure 2
ARI inhibited the caspase-3-dependent apoptosis and reversed the hepatic NAD(P)(H) contents and redox status imbalance while it lowered ROS content. (a) Representative immunostained photograph. (b)-(c) At the protein level, ARI markedly enhanced Bcl-2 and the Bcl-2/Bax ratio while it inhibited cleaved caspase 3 as compared with the Ctrl group, although there was no marked effect on Bax. (d)–(g) After ARI treatment, the I/R-induced decreases in cytoplasmic NAD and cytosolic NADPH and GSH were significantly attenuated, while cytoplasmic NADH and cytosolic NADP and MDA presented the opposite trends. (h)–(j) Remarkable increases could also be observed in the rates of NAD/NADH, NADPH/NADP, and GSH/GSSG after ARI administration. (k)-(l) In flow cytometry used to detect the proportion of ROS-positive hepatocyte, Ctrl group was significantly higher than Sham group, whereas ARI administration markedly attenuated this variation (for each condition, data are expressed as mean ± SEM and analyzed by unpaired Student’s -test; , ; ).
