Polyol Pathway Exacerbated Ischemia/Reperfusion-Induced Injury in Steatotic Liver
Figure 3
AR-overexpression worsened L02 hepatocytes’ apoptosis, elevated intracellular MDA level, and disrupted hepatocytes’ mitochondrial membrane potential. (a) After steatosis induction, L02 cell from pAR, Ctrl, and GFP group showed significantly high TG level as compared with Lean group, although there was no marked variance among pAR, Ctrl, and GFP groups. (b)-(c) On FCM using TUNEL staining, AR overexpression evidently deteriorated the H/R-induced apoptosis as compared with Ctrl and GFP group. (d) Meanwhile, pAR transfection also notably elevated the MDA contents when compared to Ctrl and GFP group. (e) In fluorescence using JC-1 staining to detect ΔΨm, the levels of Ctrl and GFP groups were approximately identical, which were clearly lower and higher than those of Sham (Red-fluorescent and cytoplasmic dominance) and pAR group (Green-fluorescent and cytomembrane dominance), respectively (for each condition, data are expressed as mean ± SEM and analyzed by unpaired Student’s -test; , ).
