LPS Induces Occludin Dysregulation in Cerebral Microvascular Endothelial Cells via MAPK Signaling and Augmenting MMP-2 Levels

Figure 3

Roles of p38MAPK and JNK signaling in LPS-induced alterations of occludin and ZO-1. hCMEC/D3 were pretreated with inhibitors of p38MAPK (SB203580, 3.85 μg/mL) or JNK signaling pathways (SP600125, 0.22 μg/mL) for 1 h prior to 24-hour LPS treatment (10 μg/mL). Protein levels of occludin (a) and ZO-1 (b) were detected by western blot. Occludin (c) and ZO-1 (d) RNA levels were measured with qRT-PCR. Data are expressed as mean ± SEM (). and versus control. ^#, ^### versus LPS.

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