LPS Induces Occludin Dysregulation in Cerebral Microvascular Endothelial Cells via MAPK Signaling and Augmenting MMP-2 Levels

Figure 4

LPS activates p38MAPK and JNK phosphorylation. hCMEC/D3 were incubated with 10 μg/mL LPS for different time periods (0, 15, 30, 60, 120, and 240 min) before phosphorylation levels of p-p38 (a) and p-JNK (b) were tested by western blot. Next, hCMEC/D3 were pretreated with SB203580 (3.85 μg/mL) or SP600125 (0.22 μg/mL) 1 h prior to 1-hour LPS exposure (10 μg/mL), and phosphorylation levels of p-p38 (c) and p-JNK (d) were detected by western blot. Results are the mean ± SEM (). , , and versus control. ^#, ^### versus LPS.

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