High Potency of a Novel Resveratrol Derivative, 3,3′,4,4′-Tetrahydroxy-trans-stilbene, against Ovarian Cancer Is Associated with an Oxidative Stress-Mediated Imbalance between DNA Damage Accumulation and Repair

Figure 5

Effect of RVT and 3,3′,4,4′-THS on incidence of apoptosis ((a)–(d)) and activity of caspases ((e)–(g)) in ovarian cancer cells. The presence of apoptotic cells was evaluated according to their staining with Hoechst 33258. Representative images showing the effect of 3,3′,4,4′-THS on apoptosis in A2780 cells (d). Apoptotic cells (marked with arrows) were identified according to the presence of condensed nuclei and characteristic cell blebbing. The activity of initiator (8 and 9) and effector (3) caspases was evaluated upon 24-h cell treatment with stilbenes using a commercially available test. The asterisks indicate a significant difference as compared to the control group. The hashes indicate a significant difference as compared to cells subjected to RVT. The experiments were performed in hexaplicate.