Enhancement of the Acrolein-Induced Production of Reactive Oxygen Species and Lung Injury by GADD34

Figure 3

GADD34 mediates acrolein-caused lung injury. The wild-type and GADD34-knockout mice were intranasally instilled by 1 and 5 μmol/kg acrolein. The mice were treated daily for 5 d/week for up to 28 days and then were sacrificed at 7 and 28 days. (a) The whole lungs of wild-type and GADD34-knockout mice were photographed at days 7 and 28. (b) H&E staining of lung tissue. Scale bar: 50 μm. Analysis of alveolar length determined by mean linear intercepts ( to 7 mice in each group) and the number of alveolar macrophages in wild-type and GADD34-knockout mice ( mice in each group). (c) Lungs stained for epithelial type II cells (ProSpC green), nuclei (DAPI blue), and the number of epithelial type II-positive cells in wild-type and GADD34-knockout mice (10 fields, ). Scale bar: 50 μm. , . Data are represented as means ± s.e.m. (d) Levels of ROS production in the lung of wild-type and GADD34-knockout mice were measured by DCFH-DA after acrolein treated.

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