The Effect of Age on Osteogenic and Adipogenic Differentiation Potential of Human Adipose Derived Stromal Stem Cells (hASCs) and the Impact of Stress Factors in the Course of the Differentiation Process

Figure 3

Effect of age on senescence and apoptosis. Quantification of senescence staining showing differences in dye absorption (a). Percentage of dead cells calculated from Calcein-AM/Propidium Iodide staining (b). Cells stained with Calcein-AM, Propidium Iodide, antibodies for caspase-3 (nuclei counterstained with DAPI) and senescence dye showing cells with β-galactosidase accumulation (c). Evaluation of p53 mRNA levels (d). Abbreviations used to describe different cell types: lc: live cell, dc: dead cell, cas: caspase-3 positive cell, and sc: β-galactosidase positive cell. Magnification ×100, scale bars: 250 μm. Results expressed as mean ± SD. value < 0.05. (>20), (>50), (>60), and (>70): donor age range.

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