Dexmedetomidine Attenuates Oxidative Stress Induced Lung Alveolar Epithelial Cell Apoptosis In Vitro

Figure 3

Dex reversed the ROS generation and cytochrome C release in A549 cells induced by H₂O₂. (a) ROS production detected by flow cytometry after DCF staining. The increase of the FITC fluorescence intensity indicated the increase in percentage of DCF positive cells; (b) percentage of the DCF positive cells, ; (c) Overexpression of cytochrome C in A549 cells was not reversed by Dex and was enhanced by atipamezole, Dex treated together; (d) mean fluorescence intensity of cytochrome C. Scale bar = 50 μm. ; . DCF: 2′-7′-dichlorodihydrofluorescein diacetate.

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