Dexmedetomidine Attenuates Oxidative Stress Induced Lung Alveolar Epithelial Cell Apoptosis In Vitro

Figure 4

Dex prevented the downregulation of p-mTOR, ERK1/2, and cell cycle arrest induced by H₂O₂, which was reversed by atipamezole. (a) Expression of p-mTOR in A549 cells assessed by immunofluorescent staining (green); (b) mean fluorescence intensity of p-mTOR; (c) expression of ERK1/2 in A549 cells assessed by immunofluorescent staining (green); (d) mean fluorescence intensity of ERK1/2. Scale bar = 50 μm; ; (e) cell cycle analyzed by flow cytometry. Dex prevented the G0/G1 arrest of A549 cells induced by H₂O₂ and attenuated by atipamezole; (f) scatter plots of mean percentage of G0/G1 cells; . , .

(a)

(b)

(c)

(d)

(e)

(f)