Toll-Like Receptor 4 Promotes NO Synthesis by Upregulating GCHI Expression under Oxidative Stress Conditions in Sheep Monocytes/Macrophages

Figure 2

LPS stimulation triggered rapid infiltration of neutrophils in transgenic sheep. (a) Pathological changes were examined microscopically (hematoxylin and eosin staining, ×400). (b) MD2 expression was detected by immunohistochemistry (×200). (c) CD14, (d) NF-κB, and (e) TLR4 transcription in mononuclear cells from G1 transgenic lambs were detected by real-time quantitative PCR. Tg: transgenic sheep, WT: wild-type. The results are expressed as mean ± SE; in Tg versus WT groups.

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