Thiosemicarbazone p-Substituted Acetophenone Derivatives Promote the Loss of Mitochondrial , GSH Depletion, and Death in K562 Cells

Figure 3

Effect of TSC compounds on mitochondrial membrane potential and nuclear morphology after exposure to TSC compounds. Simultaneous measurements of (DiOC₆(3), green fluorescence) and nuclear morphology (DAPI, blue fluorescence) were made in K562 cells after different incubation times in the absence (control) and in the presence of TSC compounds. Bleaching of the green fluorescence of DiOC₆(3) indicates the disruption of . Panel (a) shows an image from a control sample of K562 cells after 6 h incubation in the absence of TSC compounds. From left to center, panel (b) shows images of K562 cells after 3 h incubation in the presence of 0.01 or 0.1 mM of the indicated TSC compounds (i.e., TSC-Cl, TSC-Me, and TSC-F); the right side shows images of K562 cells after 1 h incubation in the presence of 1 mM of the same compounds. From left to center, panel (c) shows images of K562 cells after 3 h incubation in the presence of 0.01 or 0.1 mM of the indicated TSC compounds (i.e., TSC-H and TSC-NO₂); the right side shows images of K562 cells after 1 h incubation in the presence of 1 mM of the same compounds. Panel (d) shows images of K562 cells after 6 h incubation in the presence of 1 mM of the indicated TSC compounds, that is, TSC-Cl, TSC-Me, TSC-F, TSC-H, and TSC-NO₂.

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