Glucose Oxidase Induces Cellular Senescence in Immortal Renal Cells through ILK by Downregulating Klotho Gene Expression

Figure 1

GOx induces senescence in wild-type mouse cortical tubule (MCT) cells. MCT cells were treated with 2.5 mU/mL GOx for 24, 48, or 72 h. (a) 4-Hydroxy-2-nonenal proteins adducts were analyzed by western blot. (b) p16 and (c) p53 protein expressions were analyzed by western blot. A representative blot is shown in each case. Bar graphs represent the densitometric analysis of the bands. The results are expressed as densitometric units and are the mean ± s.e.m. from six different experiments. (d) SA-β-GAL activity was analyzed by confocal microscopy using the fluorescent substrate C12FDG. A representative experiment is shown. Bar graph represents the analysis of the fluorescence from five different experiments and the mean ± s.e.m. is expressed as fluorescence units. versus control (time 0).

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