Glucose Oxidase Induces Cellular Senescence in Immortal Renal Cells through ILK by Downregulating Klotho Gene Expression

Figure 2

GOx induces an increase in ILK protein expression and activity. MCT cells were treated with 2.5 mU/mL GOx for 24, 48, or 72 h. (a) ILK and (b) phospho-GSK3β and GSK3-β protein expression was analyzed by western blot. A representative blot is shown in each case. Bar graphs represent the densitometric analysis of the bands. The results are expressed as densitometric units and are the mean ± s.e.m. from four different experiments. versus control (time 0).

(a)

(b)