Glucose Oxidase Induces Cellular Senescence in Immortal Renal Cells through ILK by Downregulating Klotho Gene Expression

Figure 4

Antiaging gene Klotho is modulated by GOx and mediates cellular senescence induced by GOx. (a) MCT cells were treated with 2.5 mU/mL of GOx for 24, 48, and 72 h and mRNA Klotho expression was analyzed by RT and real time PCR. Bar graph represents the mean ± s.e.m. from three different experiments. MCT cells were transfected with a plasmid containing Klotho protein (pEF1-Klotho) or with an empty vector (pEF1-Empty) as control, and (b) mRNA Klotho expression was analyzed by RT and real time PCR. Bar graph represents the mean ± s.e.m. of three different experiments. (c) MTC cells transfected with Klotho protein were treated with 2.5 mU/mL of GOx for 24, 48, and 72 h and p16 protein expression was evaluated by western blot. A representative blot is shown. Bar graphs represent the densitometric analysis of the bands. The results are expressed as densitometric units and are the mean ± s.e.m. from five different experiments. versus control (time 0).

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