Glucose Oxidase Induces Cellular Senescence in Immortal Renal Cells through ILK by Downregulating Klotho Gene Expression

Figure 6

GOx induced senescence through ILK in other renal cells. HEK293T cells were treated with 2.5 mU/mL GOx for 24, 48, or 72 h. (a) ILK and p16 protein expressions were analyzed by western blot. A representative blot is shown in each case. Bar graphs represent the densitometric analysis of the bands. The results are expressed as densitometric units and are the mean ± s.e.m. from six different experiments. (b) HEK293T cells were transfected with Klotho protein (pEF1-Klotho, closed bars) or empty vector (pEF1-Empty, open bars) and then treated with 2.5 mU/mL of GOx for 24, 48, and 72 hs. p16 protein expression was evaluated by western blot. A representative blot is shown. Bar graphs represent the densitometric analysis of the bands. The results are expressed as a percentage of control and are the mean ± s.e.m. from five different experiments. versus control or time 0.

(a)

(b)