hCLOCK Causes Rho-Kinase-Mediated Endothelial Dysfunction and NF-κB-Mediated Inflammatory Responses

Figure 1

Effect of hypoxia over time on protein expression levels of hCLOCK and RhoA in HUVECs. (a) Western blot analysis was performed to evaluate Rac1 and CLOCK protein expression levels in HUVECs exposed to a hypoxic environment. Expression levels are measured at 0, 12, and 24 hours, normalized to β-actin control. (b) RhoA activation assay and Western blot analysis were performed to measure protein expression levels of activated GTPase-bound RhoA in HUVECs exposed to a hypoxic environment. Expression levels are measured at 0, 12, and 24 hours, normalized to total cellular RhoA.

(a)

(b)