OGG1 Involvement in High Glucose-Mediated Enhancement of Bupivacaine-Induced Oxidative DNA Damage in SH-SY5Y Cells

Figure 5

The levels of ROS were measured by flow cytometry. Con: SH-SY5Y cells of group control. HG: SH-SY5Y cells were exposed to 50 mM glucose for 8 d. Bup: SH-SY5Y cells were treated with bupivacaine for 24 h. HG + Bup: SH-SY5Y cells were incubated with 50 mM glucose for 8 d and then treated with bupivacaine for 24 h. HG + Bup + NAC: cells treated with 50 mM high glucose for 8 d and then pretreated with 5 mM NAC for 6 h prior to 1.5 mM bupivacaine exposure for 24 h. (a) The levels of ROS induced by high glucose or bupivacaine. High glucose enhanced ROS overproduction induced by bupivacaine. (b) NAC attenuated ROS overproduction induced by high glucose and bupivacaine. Summarized data shows the fluorescence intensity of ROS as detected by flow cytometry. Data represented are mean ± SD (), .

(a)

(b)