OGG1 Involvement in High Glucose-Mediated Enhancement of Bupivacaine-Induced Oxidative DNA Damage in SH-SY5Y Cells

Figure 7

The effect of NAC on 8-oxodG expression and DNA damage induced by high glucose and bupivacaine. Con: SH-SY5Y cells of group control. HG + Bup: SH-SY5Y cells were incubated with 50 mM glucose for 8 d and then treated with bupivacaine for 24 h. HG + Bup + NAC: cells treated with 50 mM high glucose for 8 d and then pretreated with 5 mM NAC for 6 h prior to 1.5 mM bupivacaine exposure for 24 h. (a) NAC attenuated 8-oxodG expression induced by high glucose and bupivacaine. Summarized data show 8-oxodG expression as measured by the mean fluorescence intensity. (b) NAC attenuated DNA damage induced by high glucose and bupivacaine. Summarized data show DNA damage as detected by Olive tail moment. Data are presented as mean ± SD (), .

(a)

(b)