Nrf2 Signaling and the Slowed Aging Phenotype: Evidence from Long-Lived Models

Figure 4

Nrf2 regulated protein expression in skeletal muscle from crowded litter mice. No significant sex or model differences were observed in skeletal muscle from crowded litter (CL) animals. Nrf2, NQO1, SOD-1, and Prdx1 were analyzed by western blotting and normalized to tubulin, shown below the proteins from each blot. Data are expressed as a ratio of target protein to tubulin (mean ± SEM). males and females in each condition. CL mice, 3-4 months old, were generated following the previously described procedure [5]. UM-HET3 litters were culled to eight pups (control) or supplemented by transfer of newborn mice to produce litters of 12 mice (CL). Litters were weaned at three weeks of age, after which both groups were fed ad libitum.