Upregulation of Multidrug Resistance-Associated Protein 1 by Allyl Isothiocyanate in Human Bronchial Epithelial Cell: Involvement of c-Jun N-Terminal Kinase Signaling Pathway

Figure 1

Effect of cell signaling pathway inhibitor on MRP1 mRNA expression in 16HBE14o- cells. (a) Cells were pretreated with or without PI3K/Akt inhibitor LY294002 (10 μM) 30 min or 60 min prior to the exposure to AITC for 24 h, and then the relative expression level of MRP1 was measured by RT-PCR. (b) Cells were pretreated with or without ERK inhibitor U0126 (20 μM) 30 min or 60 min prior to the exposure to AITC for 24 h, and then the relative expression level of MRP1 was measured by RT-PCR. (c) Cells were pretreated with or without JNK inhibitor SP600125 (20 μM) for 30 min or 60 min prior to the exposure to AITC for 24 h, and then the relative expression level of MRP1 was measured by RT-PCR. Significant difference from control, . Significant difference from corresponding AITC control, .

(a)

(b)

(c)