Upregulation of Multidrug Resistance-Associated Protein 1 by Allyl Isothiocyanate in Human Bronchial Epithelial Cell: Involvement of c-Jun N-Terminal Kinase Signaling Pathway
Table 1
Effect of cell signaling pathway inhibitor on MRP1 mRNA expression.
GAPDH
MRP1
ΔCt
ΔΔCt
RQ = 2−ΔΔCt
Control
15.12 ± 0.52
19.72 ± 0.50
4.60 ± 0.41
0
1
AITC
14.96 ± 0.67
16.30 ± 0.58
1.34 ± 0.17
−3.27 ± 0.34
9.79 ± 2.34
LY30
15.23 ± 0.45
19.53 ± 0.94
4.31 ± 1.03
−0.29 ± 0.72
1.33 ± 0.62
LY30 + AITC
14.85 ± 0.66
16.54 ± 0.49
1.68 ± 0.20
−2.92 ± 0.38
7.73 ± 2.13
LY60
14.97 ± 0.71
19.00 ± 1.36
4.02 ± 1.16
−0.58 ± 0.87
1.67 ± 0.90
LY60 + AITC
15.36 ± 0.76
17.51 ± 0.96
1.79 ± 0.26
−2.81 ± 0.32
7.16 ± 1.55
U30
14.79 ± 0.24
19.03 ± 1.39
4.24 ± 1.15
−0.36 ± 0.74
1.41 ± 0.78
U30 + AITC
14.83 ± 0.54
16.67 ± 1.05
1.84 ± 0.59
−2.76 ± 0.25
6.83 ± 1.11
U60
14.74 ± 0.70
18.92 ± 1.02
4.18 ± 0.64
−0.42 ± 0.35
1.37 ± 0.31
U60 + AITC
14.95 ± 0.62
16.79 ± 0.65
1.87 ± 0.37
−2.76 ± 0.23
6.84 ± 1.15
SP30
15.58 ± 0.18
23.05 ± 0.70
7.47 ± 0.79
2.87 ± 0.42
0.14 ± 0.04
SP30 + AITC
15.45 ± 0.66
19.13 ± 0.58
3.68 ± 0.51
−0.92 ± 0.17
2.26 ± 0.78
SP60
15.56 ± 0.45
22.71 ± 0.68
7.15 ± 0.30
2.54 ± 0.35
0.18 ± 0.04
SP60 + AITC
15.12 ± 0.61
19.04 ± 0.39
3.92 ± 0.81
−0.68 ± 0.55
1.68 ± 0.60
Data were normalized against control and presented mean ± S.D. Experiments were performed in triplicate. Significant difference from control, . #Significant difference from corresponding AITC control, .
