3-(3-Pyridylmethylidene)-2-indolinone Reduces the Severity of Colonic Injury in a Murine Model of Experimental Colitis

Figure 1

PMID pretreatment attenuates DSS-induced colitis in ICR mice. Following treatment with the indicated doses of PMID per os for 7 days, colitis was induced by DSS (3%) via drinking water for 7 days. During DSS exposure weight loss of the animals was recorded daily (a). Mice without DSS and PMID treatment were used as normal control (NC) and mice treated with DSS only without PMID were used as model control (MC). DAI incorporating BW loss, diarrhoea, and rectal bleeding score was assessed daily (b) (, data are expressed as mean ± SD). Following sacrifice, colons length was measured (c) and then colon samples were fixed in 4% paraformaldehyde, stained with hematoxylin/eosin, and visualized at 10x magnification (d). Representative images of severely inflammatory cell infiltration (arrowheads) and loss of colonic crypts in MC group compared to NC group. Results represented mean ± SD. /group. The statistical difference between the samples was demonstrated as or or .

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