3-(3-Pyridylmethylidene)-2-indolinone Reduces the Severity of Colonic Injury in a Murine Model of Experimental Colitis

Figure 3

PMID pretreatment lessens expression levels of proinflammatory markers in colon tissue of DSS-induced ICR mice. Following treatment with the indicated doses of PMID per os for 7 days, colitis was induced by DSS (3%) via drinking water for 7 days. Then the mice were sacrificed and the colons were excised. Total RNA was extracted and the mRNA levels of the genes indicated were analyzed using real-time PCR. The relative expression levels of genes in normal control (NC) mice were set as 1. The data were normalized to GAPDH expression. (b) Cell lysates were prepared and the protein levels of the indicated genes were measured using ELISA. Results represented mean ± SD. /group. The statistical difference between the samples was demonstrated as or .

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