3-(3-Pyridylmethylidene)-2-indolinone Reduces the Severity of Colonic Injury in a Murine Model of Experimental Colitis

Figure 6

PMID prevents DSS-induced colitis in Nrf2-dependent manner. Nrf2^−/− mice were received 22 mg PMID/kg BW per os for 7 days prior to colitis induction by 3% DSS and the weight loss was measured. Nrf2^+/+ mice were used as control. During DSS exposure weight loss of the animals was recorded daily (a). Following sacrifice, colons length was measured (b) and then colon samples were fixed in 4% paraformaldehyde, stained with hematoxylin/eosin, and visualized at 10x magnification (c). The statistical difference between the samples was demonstrated as .

(a)

(b)

(c)