AMP-Activated Protein Kinase Attenuates High Salt-Induced Activation of Epithelial Sodium Channels (ENaC) in Human Umbilical Vein Endothelial Cells

Figure 1

Effect of high sodium treatment on ENaC expression in HUVECs. ((a) and (b)) MTT assay was performed to measure the effect of high sodium concentration on cell viability. NaCl at concentrations of 10, 20, and 30 mM was, respectively, applied to the cells for (a) 24 h and (b) 48 h ( in each group). ((c)–(h)) Effects of high sodium application (additional 10, 20, and 30 mM NaCl treated for 24 h) on α-, β-, and γ-subunit levels of ENaC in HUVECs. Levels of ENaC subunits and β-actin were evaluated using western blot analysis. The densitometry values were normalized to β-actin ( in each group). indicates ; represents versus control.

(a)

(b)

(c)

(d)

(e)

(f)

(g)

(h)