Polydatin Protecting Kidneys against Hemorrhagic Shock-Induced Mitochondrial Dysfunction via SIRT1 Activation and p53 Deacetylation

Figure 2

Effect of polydatin (PD) administration on the protein expression and acetylation of p53 in the renal cortex after HS/R and in HK-2 after H/R, respectively. (a) Expression of p53 protein and (b) determination of acetyl-p53 levels on purified p53 () in the renal cortex; (c) expression of p53 protein determined using western blotting (); (d) expression of acetyl-p53 determined in purified p53 using western blotting (); (e, f) intracellular redistribution of p53 protein determined using confocal microscopy (original magnification, ×630); (g, h) expression of acetyl-p53 determined using flow cytometry. , compared with the value of the vehicle group; , compared with the value of the vehicle group; compared with the value of the PD group; compared with the value of the PD/Ex527 group. GAPDH, glyceraldehyde 3-phosphate dehydrogenase; H/R, hypoxia/reoxygenation; IP, immunoprecipitation.

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