Uncoupling of Vascular Nitric Oxide Synthase Caused by Intermittent Hypoxia

Figure 4

Uncoupled eNOS in the endothelium. Representative images of DHE staining of endothelial monolayer with and without incubation with L-NAME (20x magnification) (a). Quantification of fluorescence levels using CTCF (b). Values are displayed as mean ± SD and represent = 5-6 mice. Statistical analysis was done using Student’s -test which was used as a statistical test within groups before and after L-NAME. before and after L-NAME. Two-way repeated measures ANOVA followed by Bonferroni posttest was used for comparison between groups after L-NAME. versus IA, versus IH, and versus IADB. CTCF: corrected total cell fluorescence, DHE = dihydroethidium, and L-NAME = N_ω-nitro-L-arginine methyl ester.

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