Short-Term Effects of Chlorpromazine on Oxidative Stress in Erythrocyte Functionality: Activation of Metabolism and Membrane Perturbation

Figure 9

Phosphatidylserine exposure following treatment of RBCs for 2 h with CPZ 50 μM and t-BHT 10 μM. (a) Flow cytometry analysis of annexin-V-binding of erythrocytes following exposure to buffer solution without (A) and with CPZ (B) or t-BHT (C); (b) FACS histogram showing annexin binding in a representative experiment of RBCs incubated with CPZ; and (c) arithmetic means ± SD of erythrocyte annexin-V-binding ( = 12) following incubation to buffer solution without (A) or with (B) presence of CPZ; as a control, the effect of BHT is shown (C). ignificant differences at versus control.

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