Adiponectin Protects against Glutamate-Induced Excitotoxicity via Activating SIRT1-Dependent PGC-1 Expression in HT22 Hippocampal Neurons

Figure 3

The APN peptide decreased Glu-induced oxidative stress. (a) HT22 cells were treated with the APN peptide at the indicated concentrations (100 μM, 250 μM, and 500 μM) for 6 h and then exposed to 5 mM Glu for 24 h, followed by incubation with 10 μM DCF-DA for 30 min. ROSs were stained with green fluorescence. (b) ROS levels. (c) MDA levels. (d) SOD activities. (e) GSH-Px levels. The results are expressed as the mean ± SD. ; versus the control group; versus the Glu group; versus the 100 μM APN peptide pretreatment group; versus 250 μM APN peptide pretreatment group. APN, adiponectin; OD, optical density; LDH, lactate dehydrogenase; ROS, reactive oxygen species.

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