Adiponectin Protects against Glutamate-Induced Excitotoxicity via Activating SIRT1-Dependent PGC-1 Expression in HT22 Hippocampal Neurons

Figure 4

APN peptide pretreatment increased the expression of SIRT1 and PGC-1α. (a, b, and c) Western blot analysis of the effects of the APN peptide on SIRT1 and PGC-1α expression in HT22 cells exposed to Glu. (d and e) Immunofluorescence images of SIRT1 (green)/DAPI (blue) and PGC-1α (green)/DAPI (blue) stained neuronal cells after 6 h pretreatment of the APN peptide (500 μM) and exposure to 5 mM Glu for 24 h. (f) SIRT1 expression level. (g) PGC-1α expression level. The results are expressed as the mean ± SD. ; versus the control group; versus the Glu group; versus the 100 μM APN peptide pretreatment group; versus 250 μM APN peptide pretreatment group. APN, adiponectin; SIRT1, silent information regulator 1; PGC-1α, peroxisome proliferator-activated receptor gamma coactivator 1-alpha.

(a)

(b)

(c)

(d)

(e)

(f)

(g)