High-Fat Diet Induces Oxidative Stress and MPK2 and HSP83 Gene Expression in Drosophila melanogaster

Figure 6

Expression levels of enzymes ACSL1 and ACeCS1 in response to the treatment of D. melanogaster with a regular diet (RD) and a high-fat diet (HFD) for seven days. After feeding the flies for seven days with different diets, they were homogenized and the proteins were separated by SDS-PAGE and transferred to nitrocellulose membrane. We quantified total content of proteins using specific antibodies. (a) The upper panel is a Western Blot showing expression levels of Acyl-CoA Synthetase (ACSL1) and Acetyl-CoA Synthetase (ACeCS1) with respective contents of β-actin. (b) The graphs are showing the ratio of OD from quantification of immunoreactive bands/β-actin and represent an average ± standard deviation. Three sets of forty flies were used for each diet condition. indicates a significant difference between the RD and the HFD ().

(a)

(b)