Research Article

Parishin from Gastrodia elata Extends the Lifespan of Yeast via Regulation of Sir2/Uth1/TOR Signaling Pathway

Table 1

Strains used in this study.

StrainsGenotypeSource or reference

K6001MATa, ade2-1, trp1-1, can1-100, leu2-3, 112, his3-11, 15, GAL, psi+, ho::HO::CDC6 (at HO), cdc6::hisG, ura3::URA3 GAL-ubiR-CDC6 (at URA3)[18, 35]

Δuth1 of K6001Replace the UTH1 gene in K6001 with kanamycin geneConstructed by Professor Akira Matsuura

Δskn7 of K6001Replace the SKN7 gene in K6001 with kanamycin geneConstructed by Professor Akira Matsuura

Δsod1 of K6001Replace the SOD1 gene in K6001 with kanamycin geneConstructed by Professor Akira Matsuura

Δsod2 of K6001Replace the SOD2 gene in K6001 with kanamycin geneConstructed by Professor Akira Matsuura

BY4741MATa, his3Δ1, leu2Δ0, met15Δ0, ura3Δ0[36]

Δuth1 of BY4741Replace the UTH1 gene in BY4741 with kanamycin geneConstructed by Professor Akira Matsuura

Δuth1, sir2 of BY4741Replace the UTH1 gene and SIR2 gene in BY4741 with kanamycin geneConstructed by Professor Akira Matsuura