Apigenin Attenuates Oxidative Injury in ARPE-19 Cells thorough Activation of Nrf2 Pathway

Figure 3

Apigenin increases the expression of Nrf2 target genes involved in antioxidant system in ARPE-19 cells. (a) Real-time PCR analyses of the mRNA expression of HO-1, NQO1, and GCLM in t-BHP-treated ARPE-19 cells. Significance: versus control, versus control, versus t-BHP, and versus t-BHP. (b) Real-time PCR analyses of the mRNA expression of HO-1, NQO1, and GCLM in ARPE-19 cells in the absence of t-BHP. Significance: versus control siRNA. (c) Western blot analyses of protein abundance of HO-1, NQO1, and GCLM in t-BHP-treated ARPE-19 cells with quantification. Significance: versus control, versus t-BHP, and versus t-BHP. (d) Western blot analyses of protein abundance of HO-1, NQO1, and GCLM in ARPE-19 cells in the absence of t-BHP with quantification. Significance: versus control; versus control.

(a)

(b)

(c)

(d)