Comparative Analysis of the Interaction between Different Flavonoids and PDIA3

Table 1

Stern-Volmer constant (), bimolecular quenching rate constant (), number of binding sites (), binding constant (), and dissociation constant () of several flavonoid-PDIA3 systems.

Flavonoid	(M^−1)	(M^−1 s^−1)		(M^−1)	(M)
Flavone
Trimethoxyflavone
Eupatorin-5-methyl ether
Eupatorin
Apigenin
Apigenin-7-glucoside
Luteolin-7-glucoside
Flavanone
Naringenin
Isoflavone
Genistein
Flavonol
Rhamnetin
Isorhamnetin
3-O-Methyl-quercetin
Fisetin
Kaempferol
Morin
Quercetin
Quercitrin
Isoquercetin
Rutin
Anthocyanin
Cyanidin

Data were calculate from fluorescence quenching analysis using PDIA3 in reduced conditions (pH 7.4, 25°C) and increasing concentration (0 to ) of different flavonoids. are reported as mean and standard deviation of at least three independent experiments. The number of binding sites (), the binding constant (), and the dissociation constant () were estimated using the equation described by Bi et al. [31] and the reiterative calculation process described by Sun et al. [32].