Thioredoxin Binding Protein-2 Regulates Autophagy of Human Lens Epithelial Cells under Oxidative Stress via Inhibition of Akt Phosphorylation

Figure 5

Knockdown of TBP-2 attenuated the autophagic response in LECs under oxidative stress. (a) Knockdown of TBP-2 successfully inhibited TBP-2 expression to about 18%. (b) Twenty-four hours after transfection, cells were treated with H₂O₂ (50 μM) for 12 hours. Western blot showed that si-TBP-2 cells had lower LC3-II and p62 level than NC cells. (c) Immunofluorescent detection of LC3 indicated lower intensity in the cytoplasm of si-TBP-2 cells than NC cells. (d) Cytotoxicity tests revealed that, after 12 and 24 hours of H₂O₂ treatments, no significant difference was found in si-TBP-2 cells and NC cells. Scale bar, 25 nm. .

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