Calcium Oxalate Induces Renal Injury through Calcium-Sensing Receptor

Figure 2

CaSR and MAPK expression in HK-2 cells in the different groups, as determined by western blotting. (1) Control group: HK-2 cells were continuously cultured at 37°C for 6 h in DMEM. (2) COM group: HK-2 cells were incubated with COM (67 μg/cm² of cells) at 37°C for 6 h. (3) COM+GdCl₃ group: HK-2 cells were pretreated with the CaSR activator GdCl₃ (300 μM) for 30 min, and then COM crystals were added to the growth medium (67 μg crystals/cm² of cells) at 37°C for 6 h. (4) COM+NPS2390 group: HK-2 cells were pretreated with the CaSR inhibitor NPS2390 (10 μM) for 60 min, and then COM crystals were added to the growth medium (67 μg crystals/cm² of cells) at 37°C for 6 h. The data are expressed as the mean ± SEM. compared with the control group and compared with the COM group .

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