Pharmacological Inhibition of NLRP3 Inflammasome Attenuates Myocardial Ischemia/Reperfusion Injury by Activation of RISK and Mitochondrial Pathways

Figure 4

Prosurvival RISK pathway activation in the rat hearts exposed to 30 min ischemia plus 20 or 60 min reperfusion, pretreated or not with INF4E. (a) Representative Western blotting for cardiac levels of total ERK1/2 expression and Thr202/Tyr204 and Thr185/Tyr187 phosphorylation, respectively, total Akt protein expression and Ser473 phosphorylation, and total GSK-3β protein expression and Ser9 phosphorylation, performed on heart extracts. (b)–(d) Histograms report densitometric analysis of the phosphorylated-to-total form ratio. Data are means of 6 rats ± SEM. , , and versus Sham; , , and versus IR.