Notch1 Pathway Protects against Burn-Induced Myocardial Injury by Repressing Reactive Oxygen Species Production through JAK2/STAT3 Signaling

Figure 4

Notch blockade leads to increased ROS production. Rat cardiomyocytes were challenged with burn serum in vitro in the presence of DMSO or GSI. ROS were examined by way of FACS (a) and were quantified by way of mean fluorescence intensity (b). RBP-J KO or control mice were subjected to burn injury and were examined 12 hours after injury. (c) Myocardium tissues were isolated and examined for superoxide anions content by way of lucigenin-enhanced luminescence. The values presented are the mean ± SEM ( per group). .

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(b)

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