The Effect of Leonurus sibiricus Plant Extracts on Stimulating Repair and Protective Activity against Oxidative DNA Damage in CHO Cells and Content of Phenolic Compounds

Figure 2

DNA damage and repair in CHO cells with 0.05 mg/mL (a), 0.1 mg/mL (b), and 0.5 mg/mL (c) of L. sibiricus AP, R in vitro plant and AP, R in vivo plant extracts. DNA damage (strand breakage) was induced or not by H₂O₂ at a concentration of 75 μM at 4°C on ice and measured as a percentage of the tail DNA in the alkaline comet assay. by comparing AP in vitro plant with AP in vivo plant extracts and R in vitro plant with R in vivo plant extracts at the appropriate time of incubation. The number of cells scored for each individual was 100. Data is expressed as means ± SE of at least three independent experiments.