Antioxidant and Vasodilator Activity of Ugni molinae Turcz. (Murtilla) and Its Modulatory Mechanism in Hypotensive Response

Figure 2

Antioxidant analysis of Murtilla in endothelial cells. (a) Effects of increasing concentrations of Murtilla (0.01–44 μgGAE/mL) upon ROS production, measured by the chemiluminescent reaction of luminol. (b) Effects of increasing concentrations of Murtilla (0.01–44 μgGAE/mL) on reduction of lipid peroxidation induced by H₂O₂, measured by BODIPY C-11 dye; (b1) control; (b2) 0.01 μgGAE/mL; (b3) 1 μgGAE/mL; and (b4) 44 μgGAE/mL: the oxidized fraction is shown. (c) Effects of increasing concentrations of Murtilla (0.01–44 μgGAE/mL) on reduction of intracellular superoxide anion () produced by H₂O₂, measured by dihydroethidium dye; (c1) control; (c2) 0.01 μgGAE/mL; (c3) 1 μgGAE/mL; (c4) and 44 μgGAE/mL: the oxidized fraction is shown. All assays were performed in quintuplicate of three independent experiments, and dose-response curves were plotted using a nonlinear regression model.