Antioxidant Effects of Sheep Whey Protein on Endothelial Cells

Figure 1

(a) Effects of whey protein on viability of EA.hy926 cells presented as % of control (untreated cells). (b) Effects of tBHP on viability of EA.hy926 cells presented as % of control (untreated cells). (c) The histogram of cell counts versus fluorescence of 10,000 cells analyzed by flow cytometer for the detection of GSH in EA.hy926 cells treated with tBHP at 0.15 and 0.3 mM for 1 h. FL2 represented the detection of fluorescence using 488 and 580 nm as the excitation and emission wavelength, respectively. (d) GSH levels in EA.hy926 cells treated with tBHP at 0.15 and 0.3 mM for 1 h, presented as % of control. (e) The histogram of cell counts versus fluorescence of 10,000 cells analyzed by flow cytometer for the detection of GSH in EA.hy926 cells treated with tBHP at 0.15 and 0.3 mM for 1/2 h. (f) GSH levels in EA.hy926 cells treated with tBHP at 0.15 and 0.3 mM for 1/2 h, presented as % of control. All values are presented as the mean ± SEM of 3 experiments ( for cell viability assay; for GSH assay). Statistically significant compared to tBHP alone ().

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