Antioxidant Effects of Sheep Whey Protein on Endothelial Cells

Figure 2

Effects of whey protein on GSH and GSSG levels in EA.hy926 cells. (a) The histogram of cell counts versus fluorescence of 10,000 cells analyzed by flow cytometer for the detection of GSH. FL2 represented the detection of fluorescence using 488 and 580 nm as the excitation and emission wavelength, respectively. (b) GSH levels in EA.hy926 cells presented as % of control. (c) GSSG levels as evaluated by spectrophotometer. Cells were studied under three conditions: under normal conditions (control), under treatment with tBHP (0.3 mM) for 1 h, and under the combination of whey protein (0.78–6.24 mg of protein mL⁻¹) for 24 h and tBHP (0.3 mM) for 1 h. All values are presented as the mean ± SEM of 3 experiments (). Statistically significant compared to tBHP alone (). ^#Statistically significant compared to control ().

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