Research Article
Cholesterol Retards Senescence in Bone Marrow Mesenchymal Stem Cells by Modulating Autophagy and ROS/p53/p21Cip1/Waf1 Pathway
Figure 4
Activity of SA-β-gal in BMSCs with RITA (p53 activator) or 3-MA (autophagy inhibitor). (a) Representative image of SA-β-gal staining in the P3 BMSCs treated with RITA a p53 activator (1.0 μM), CH (15 μg/mL), or RITA + CH. (b) Percentage of SA-β-gal positive cells in different groups shown in (a). for each group, versus Ctl group; versus RITA group. (c) Representative image of SA-β-gal staining in the P3 BMSCs treated with 3-MA an autophagy inhibitor (5.0 mM), CH (15 μg/mL), or 3-MA + CH. (d) Percentage of SA-β-gal positive cells in different groups shown in (c). for each group, versus Ctl group; versus 3-MA group.
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