Resveratrol Enhances Autophagic Flux and Promotes Ox-LDL Degradation in HUVECs via Upregulation of SIRT1

Figure 3

Resveratrol ameliorates Ox-LDL-induced impaired autophagic flux and reduces the accumulation of Ox-LDL in HUVECs. Western blotting analysis of SIRT1, LC3-II, and p62 after HUVEC pretreatment with 50 μM resveratrol for 1 h and subsequently incubation with Ox-LDL (60 μg/mL) for 12 h (a, b). Data are expressed as mean ± SEM; ; , , and versus controls; NS: not significant. (c) The accumulation of Dil-Ox-LDL in HUVECs was determined by confocal microscopy. Scale bar: 100 μm. (d) Flow cytometry detected the fluorescence density of Dil-Ox-LDL in HUVECs. (e) Western blotting analysis of LOX-1 after cells was treated with Ox-LDL or RSV; data are expressed as mean ± SEM; ; versus control; NS: not significant.

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