Hepatitis C Virus NS5A Protein Triggers Oxidative Stress by Inducing NADPH Oxidases 1 and 4 and Cytochrome P450 2E1

Figure 1

Domain I of HCV NS5A protein is responsible for induction of oxidative stress in Huh7 cells. (a) Schematic representation of truncated forms of NS5A used in the study. Note that all NS5A expression constructs contained an N-terminal c-myc tag. The obtained plasmids were transfected into Huh7 cells, which were subjected to detection of NS5A by Western-blot 48 h after transfection using an anti-myc antibody (b) or to quantification of ROS levels in H₂DCFDA (c) or DHE (d) assays as described in Section 2. (c and d) Results are presented as mean ± SD from seven replicates. versus pVax1 (ANOVA with Tukey-Kramer post hoc analysis).

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